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. 2020 Dec 17;8:616771. doi: 10.3389/fcell.2020.616771

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Copyright © 2020 Zhang, Ding, Ahmad, Wang, Duan, Miao, Xiong and Huo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BPB exposure increased DNA damage in mouse oocytes. (A) Images depicting DNA damage in control and BPB-treated oocytes. γ.H2A.X, red; DNA, blue. Bar, 20 μm. (B) Quantitative analysis of the fluorescence intensity of γ.H2A.X in control and BPB-treated oocytes. Control, n = 33; BPB, n = 32. ^∗∗Significantly different (P < 0.01). (C) ROS depiction in control and BPB-exposed oocytes. ROS, red. Bar, 100 μm. (D) ROS fluorescence intensity in control and BPB-exposed oocytes. Control, n = 37; BPB, n = 36. No significant difference (P > 0.05). (E) The ATP content in control and BPB-treated oocytes. Control, n = 30; BPB, n = 30. No Significantly (P > 0.05).