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. 2020 Dec 17;11:564833. doi: 10.3389/fphar.2020.564833

FIGURE 2.

FIGURE 2

Verifying the potential binding of HSPA8 with OC. A Coomassie blue staining of HSPA8. (A) total of 3 µg of purified HSPA8 was loaded for SDS-PAGE followed by Coomassie blue staining. (B) Biacore SPR analysis of HSPA8 and OC binding. HSPA8 was immobilized on the CM5 chip and different concentrations of OC passed through the chip for 60 s. The curves indicate the potential binding between OC and HSPA8. (C) The KD value was calculated from Biacore SPR data. (D) ITC assay of HSPA8 and OC was performed at 25°C. The raw ITC data for injecting OC into the sample cell containing HSPA8 protein. The reaction heat of OC and protein binding was expressed as differential power (DP) between the reference and sample cells. (E) Experimental data are expressed with solid dots and fitted to a binding curve by a one-site binding model. (F) Thermal shift assay of HSPA8 and OC. HSPA8 was incubated with different concentrations of OC, and the melting curve was plotted and calculated using fluorescence intensity.

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