FIGURE 4.

Verifying the potential binding of HSPA8 with OC_biotin. (A) Biacore SPR analysis of HSPA8 and OC_biotin potential binding. HSPA8 was immobilized on the CM5 chip and different concentrations of OC_biotin were passed through the chip for 60 s. The curves indicated the potential binding between OC_biotin and HSPA8. (B) The KD value was calculated from Biacore SPR data. (C) ITC measurement of HSPA8 and OC_biotin was carried out at 25°C. The raw ITC data for injecting OC_biotin into the sample cell containing HSPA8 protein. The reaction heat of OC_biotin and protein binding were expressed as differential power (DP) between the reference and sample cells. (D) Experimental data are expressed with solid dots and fitted to a binding curve by a one-site binding model.