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. 2020 Dec 17;11:564833. doi: 10.3389/fphar.2020.564833

FIGURE 8.

FIGURE 8

OC induces apoptosis of A549 cells by regulating HSPA8 protein. (A) Detection of the HSPA8 protein expression level in A549 cells by western blot analysis after 48 h of transfection, GAPDH was used as loading control. A549 cells were transfected with small interfering RNA (siRNA) and pCDNA_HSPA8 plasmids respectively for 48 h siRNA control (NC) and pCDNA3.1 vector (Vehicle) as a control. (B) The effects of OC on A549 cell viability after treatment with HSPA8 siRNA or pCDNA_HSPA8 plasmid. The cells were transfected with siRNA or plasmid for 48 h and OC was supplemented at the indicated concentrations for 72 h. Cell viability was then detected using the CCK-8 Assay. Data are expressed as mean ± standard deviation of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001 vs. control). (C) OC induces apoptosis of A549 cells by targeting HSPA8 protein. A549 cells were transfected with HSPA8 siRNA or plasmid for 48 h, 10 µM OC was supplemented for another 24 h. The cells were collected and stained with Annexin V and PI for flow cytometry analysis. (D) The result of statistical analysis of apoptotic cells. Data are expressed as mean ± standard deviation of three independent experiments (**p < 0.01, ***p < 0.001 vs. OC control group).