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. 2020 Dec 17;8:609942. doi: 10.3389/fchem.2020.609942

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Copyright © 2020 D'Agostino, Motta, Romagnoli, Orlando, Tiano, La Teana and Di Marino.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) S. solfataricus growth in the presence of different GC7 concentrations. S. solfataricus cells were grown in the absence and in the presence of 10, 50, 100, 250, and 500 μM of GC7. Growth was monitored by OD600 measurements at the indicated time points. (B) Western blot analysis for the detection of hypusinated aIF5A in S. solfataricus cells after 48 h of GC7 treatment. Lane 1, control without GC7; lanes 2–6, cells grown with the indicated GC7 concentration; Lane 7, recombinant His-tagged-aIF5A. The two membranes stained with Ponceau are reported. (C) Graph reporting the ratio between hypusinated over total aIF5A signals.