Table 1.
Gametocyte, oocyst, and sporozoite production of Pf-PvCSP and WT NF54 lines.
| Parasites | Gams (%) male/female mean (SD) a | Exfl. No mean (SD) b | Exfl. Males (%)Mean (SD) c | Infected/dissected mosquitoes | Oocysts No mean (SD) d | Spz No mean e | |
|---|---|---|---|---|---|---|---|
|
WT
NF54 |
exp. 1 | 0.45/0.57 | 366 | 80 | 16/18 | 62 | 32 K |
| exp. 2 | 0.47/0.67 | 401 | 85 | 9/12 | 47 | 21 K | |
| exp. 3 | 0.52/0.63 (0.03/0.04) |
201 (87) |
38 (21) |
11/12 | 40 (9.3) |
54 K | |
| Pf-PvCSP | |||||||
| Clone 1 | exp. 1 | 0.8/1.1 | 623 | 76 | 16/16 | 44 | 17 K |
| Clone 3 | exp. 1 | 0.6/1.1 | 696 | 100 | 16/16 | 63 | 18 K |
| exp. 2 | 0.3/0.4 | 641 | 100 | 18/18 | 52 | 20 K | |
| exp. 3 | 0.7/1.0 (0.2/0.3) |
485 (89) |
65 (17) |
9/9 | 48 (6.4) |
46 K | |
Mean percentage of stage V male and female gametocytes (per 100 red blood cells) in day 14 gametocyte cultures in one to three experiments (exp.) with standard deviation (SD).
Mean number of exflagellating male gametocytes (per 105 red blood cells) at 10–20 min after activation in day 14 gametocyte cultures.
Mean percentage of exflagellating stage V male gametocytes in day 14 gametocyte cultures (1–3 exp.) with standard deviation (SD).
Mean number of oocyst per mosquito at days 10 to 12 after feeding (1–3 exp. per line; 10–30 mosquitoes per exp.).
Mean number of salivary gland sporozoites per mosquito at days 18–24 after feeding. Range corresponds to the mean number of sporozoites in multiple experiments (1–3 exp. per line; 50–90 mosquitoes per exp.). See Figure S3 for a graphic representation of the data of independent experiments.