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Cisplatin-induced autophagy in kidney proximal tubular cells. Rat kidney proximal tubular cells transiently transfected with GFP-LC3 were incubated with 20 μM cisplatin for 0–16 h to record GFP-LC3 signal (A), assess% cells with punctate/autophagic GFP-LC3 staining and % cells with apoptotic morphology (B), and analyze LC3-II formation by immunpblottinh (C). This figure was modified from Periyasamy-Thandavan et al. (2008).
