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. 2020 Apr 3;26:111–120. doi: 10.1016/j.jot.2020.03.005

Figure 2.

Figure 2

High-yield exosome production from RCCS. (A) Yield of exosomes isolated by RCCS at different rotational speeds (n = 12). Exosome yield = the number of exosome measured by Nanosight/the number of cells. (B) Particle purity of exosomes at different rotational speeds ( = 12). Particle purity = the number of particles/exosomal protein (μg). (C) Yield of exosomes isolated by RCCS at 36 rpm/min from different culture times (n = 4). (D) Average diameter of particles isolated by RCCS at 36 rpm/min from different culture times (n = 5). (E) Morphology of exosomes under transmission electron microscopy after 48∗5 h, scale bar: 200nm. Plots show yield for each method and the mean ± SD of all measurements. ∗p < 0.05, ∗∗p < 0.01, n = 3.