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. 2020 Apr 3;26:111–120. doi: 10.1016/j.jot.2020.03.005

Figure 4.

Figure 4

Exosomal LncRNA H19 in mechanical environment enhances proliferation and inhibits apoptosis of chondrocytes. (A) The expression level of LncRNA H19 in exosomes under mechanical environment detected by qRT-PCR. (B) The expression level of LncRNA H19 in U-MSCs treated with different siRNAs. (C) The proliferation was assessed by CCK-8 assay. (D–E) The protein and mRNA level of genes associated with proliferation (PCNA, Cyclin D1) and matrix synthesis (Col II, Sox 9, MMP 13, ADMST5). (F) Toluidine blue staining and the quantification of chondrocytes treated with different kinds of exosomes. (G) The quantification of toluidine blue staining. (H) Chondrocytes were pretreated with si-Exos and S-Exos followed by IL-1β (10 ng/mL) challenge for 24 h. Apoptosis was assessed by flow cytometry. (I) Apoptotic rate of chondrocytes. (J–K) The protein and mRNA level of genes associated with anti-apoptosis (Bcl-2, Bax). Data represent mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, n = 3; #p < 0.05, ##p < 0.01 compared to each other group, n = 3.