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. 2020 Oct 20;20:122–132. doi: 10.1016/j.omtm.2020.10.010

Figure 4.

Figure 4

Comparison of Gene Transfer Efficiency in C57/BL6 Mice using a LacZ Vector

(A) Quantification of B-galactosidase (LacZ) activity in homogenized tissue samples of various organs in 8-week-old C57/Bl6 mice transduced by AAV5-CB-LacZnls, MV1-CB-LacZnls, MV18-CB-LacZnls, MV20-CB-LacZnls, MV50-CB-LacZnls, and MV53-CB-LacZnls. Mice in each group (n=4) were injected with 1e12 vg via tail-vein injection. Tissues were collected 4 weeks post injection. Tissue samples were homogenized and assayed for LacZ activity. LacZ activity was normalized by protein concentrations for each tissue sample. A total of five organs were quantitated for LacZ activity: the liver, lungs, heart, gastrocnemius (GAS), and brain. Data are shown as mean values ± SD. (B) Quantification of LacZ activity in homogenized mouse liver tissue. Data are shown as mean values ± SD. ∗p < 0.05. (C) Quantification of LacZ activity in homogenized mouse lung tissue. Data are shown as mean values ± SD. ∗p < 0.05. ∗∗p < 0.01. ∗∗∗p < 0.001.