Table 1.
Summary of quantitative estimation of morphological and biophysical properties of different nanodomains obtained through STED microscopy
| Category/parameter | Length (μm) | Area (μm2) | Intensity (a.u.) | Mean normalized intensity‡ | Median normalized intensity¶ |
|---|---|---|---|---|---|
| Nanodomainβ | 0.155 ± 0.001 (0.139,0.116–0.170) | 0.0148 ± 0.0001 (0.0128, 0.0091–0.0179) | 414.88 ± 3.37 (396.90,288.63–529.46) | 1.00 ± 0.008 | (1.00, 0.73–1.33) |
| Nanodomainγ | 0.143 ± 0.001 (0.126, 0.097–0.162) | 0.0129 ± 0.0001 (0.0106, 0.0063–0.0164) | 283.77 ± 3.21 (228.69,106.81–407.36) | 1.00 ± 0.011 | (1.00, 0.47–1.78) |
| Nanodomainβ/PSD | 0.174 ± 0.004 (0.152, 0.121–0.194) | 0.0174 ± 0.0005 (0.0148, 0.0098–0.0218) | 471.90 ± 9.36 (460.70,320.80–597.70) | 1.13 ± 0.022 | (1.16, 0.81–1.51) |
| Nanodomainβ/EZ | 0.151 ± 0.003 (0.136, 0.113–0.166) | 0.0141 ± 0.0004 (0.0122, 0.0087–0.0174) | 381.60 ± 8.47 (355.80, 255.20–477.70) | 0.92 ± 0.020 | (0.90, 0.64–1.20) |
| Nanodomainγ/PSD | 0.132 ± 0.002 0.112, 0.086–0.157) | 0.0111 ± 0.0002 (0.0080, 0.0049–0.0147) | 140.30 ± 3.12 (108.80, 77.20–162.60) | 0.49 ± 0.011 | (0.48, 0.34–0.71) |
| Nanodomainγ/EZ | 0.158 ± 0.002 (0.142, 0.115–0.175) | 0.0154 ± 0.0002 (0.0133, 0.0090–0.0187) | 401.00 ± 5.52 (365.40, 254.20–513.50) | 1.41 ± 0.019 | (1.59,1.11–2.25) |
| Nanodomainβ/APP | 0.161 ± 0.003 (0.145, 0.118–0.177) | 0.0158 ± 0.0003 (0.0140, 0.0096–0.0194) | 398.00 ± 7.60 (371.40, 253.80–514.00) | 0.95 ± 0.018 | (0.94, 0.64–1.30) |
| Nanodomainγ/APP | 0.129 ± 0.001 (0.116, 0.092–0.147) | 0.0107 ± 0.0002 (0.0085, 0.0056–0.0134) | 138.10 ± 1.85 (125.20, 88.53–170.20) | 0.48 ± 0.006 | (0.55, 0.38–0.74) |
| Nanodomainβ/γ | 0.164 ± 0.004 (0.147, 0.123–0.188) | 0.0168 ± 0.0005 (0.0146, 0.0103–0.0213) | 521.83 ± 11.24 (517.48, 376.88–652.30) | 1.25 ± 0.027 | (1.30, 0.95–1.65) |
The values indicated are Mean ± SEM while values in brackets represent the median, IQR from 25% percentile to 75% percentile, ‡/¶ normalized with respect to the mean/median of the global β-secretase nanodomain intensity for nanodomainβ, nanodomainβ/PSD, nanodomainβ/EZ, nanodomainβ/APP, nanodomainβ/γ or with the mean/median of the global γ-secretase nanodomain intensity for nanodomainγ, nanodomainγ/PSD, nanodomainγ/EZ and nanodomainγ/APP.