TABLE 1.

Overview of the genetic mutations found by whole-genome sequencing of mutants resistant to 2CCA-1^a

Strain	Nucleotide mutation	Amino acid change	Locus tag	Protein function
BHN848	A344-	Frameshift-stop codon	SP_0416	FabT, MarR family transcriptional regulator
	A1601C	D534A	SP_1221	Type II restriction endonuclease
	G253-	Frameshift	SP_RS08765	Glycosyltransferase family 2 protein
	G992A	G331D	SP_1901	RNA methyltransferase
BHN849	C2030A	A677E	SP_0498	YSIRK-type signal peptide-containing protein
	G808T	E270*	SP_0742	FakB3, DegV family protein
BHN850	T33G	C11W	SP_0742	FakB3, DegV family protein
	T75C	N25N	SP_1493	MucBP domain-containing protein
	A1896491C			Noncoding region
BHN851	A1094T	D365A	SP_0686	Bacteriocin-associated integral membrane family protein
	700052–704058; 4,006-bp deletion		SP_0737–SP_0742
	G601T	A201S	SP_1706	Hypothetical protein
	G1821T	K607N	SP_1960	DNA-directed RNA polymerase subunit beta
BHN852	A625T	K209*	SP_0742	FakB3, DegV family protein
	G127A	E43K	SP_1136	DnaD domain protein
BHN857	300–396; 97-bp deletion		SPD_0646	FakB3, DegV family protein
BHN858	T386A	V129E	SPD_0646	FakB3, DegV family protein
	T222C	S74S	SPD_0453	Restriction endonuclease subunit S
BHN859	A226G	K76E	SPD_0379	FabT, MarR family transcriptional regulator
	C947T	A316V	SPD_0320	Glycosyltransferase family 1 protein
	C1169T	A390V	SPD_0709	DNA gyrase subunit B
BHN860	300–396; 97-bp deletion		SPD_0646	FakB3, DegV family protein
BHN870	665175–666813; 1,638-bp deletion	Frameshift-Stop codon	SPD_0645-SPD_0646

^aBoldface indicates genes or genetic regions affected by mutations in more than one resistant mutant.