FIG 1.
DNA damage induced a change in Rad53 mobility in S. cerevisiae but not C. glabrata. (A) Alkylating damage (MMS) and oxidative damage (H2O2) induced a shift in the mobility of ScRad53 but not CgRad53. Both conditions induced DNA damage in both species, as evidenced by increased abundance of γH2A.X. (B) MMS induced an increase in γH2A.X abundance by 20 min postexposure but did not induce even a transient shift in CgRad53 mobility. (C) MMS treatment induced DNA damage, as reflected by γH2A.X levels, to similar extents in S. cerevisiae and C. glabrata. Results were calculated from at least three independent biological replicates for every condition. In panels A and C, the cells were exposed to the indicated DNA damaging agent for 1 h.