TABLE 2.
PCR primers
| Primer name | MCV genome positiona | Sequence |
|---|---|---|
| circMCV-T_DP1F (DP1) | 1514–1533 | TACAAGCACTCCACCAAAGC |
| circMCV-T_DP1R1 (DP1R1) | 926–946 | TATTCGTATGCCTTCCCG |
| circMCV-T_DP1R2 (DP1R2) | 1046–1067 | GGACCCATACCCAGAGGAAGAG |
| circMCV-T_DP2F (DP2) | 1409–1428 | TGGTGAAGGAGGAGGATCTG |
| circMCV-T_DP2R (DP2) | 1483–1503 | GCTCTGCAAGCTCTGCTAGTT |
| circMCV-T_DP3F (DP3) | 1079–1099 | CCAGGCTTCAGACTCCCAGTC |
| circMCV-T_DP3R (DP3) | 1057–1078 | GACGCTGAGAAGGACCCATACC |
| circSMARCA5_F | NA | CTCCAAGATGGGCGAAAGT |
| circSMARCA5_R | NA | TTCTGATCCACAAGCCTCC |
| U6_F | NA | GTGCTCGCTTCGGCAGCACA |
| U6_R | NA | AAAATATGGAACGCTTCACGA |
| Linear sT_F | 397–422 | AAGCTCAGAAGTGACTTCTCTATGTT |
| Linear sT_R | 613–632 | TCTCCCCACGTCAGACAGTT |
| Linear LT + 57 kT_R (CP-R) | 3052–3080 | TTATTGAGAAAAAGTACCAGAATCTTGGG |
| Linear β-actin_F | NA | CACACTGTGCCCATCTATGAGG |
| Linear β-actin_R | NA | TCGAAGTCTAGGGCGACATAGC |
| Circ_MCV_LT_PacI F | NA | CGCTTAATTAATTTCCCATCTAGGTTGAC |
| Circ_MCV_LT_SacII R | NA | GATCCGCGGACTTACTGTTTTATTACT |
a
NA, not applicable.