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. 2020 Dec 17;11:513383. doi: 10.3389/fimmu.2020.513383

Figure 1.

Figure 1

BFRF1 suppresses SeV-mediated activation of IFN-β and ISRE promoters. Vector, BFRF1-Flag, or BGLF4-HA expression plasmid was co-transfected with pRL-TK control plasmid and IFN-β-Luc (A) or ISRE-Luc (B) reporter plasmid into HEK293T cells. At 24 h post-transfection, cells were infected with 100 HAU/ml of SeV for 16 h. Cell lysates were then collected, and luciferase activity was measured by DLR. The expression of BFRF1 or BGLF4 protein was also detected by WB using anti-Flag or anti-HA mAb, and β-actin was used to verify equal loading of protein in each lane. (C) Expression plasmid BFRF1-Flag, BGLF4-HA or vector was transfected into HEK293T cells, at 24 h post-transfection, cells were mock-infected or infected with 100 HAU/ml SeV for 16 h. Cells were then lysed and RNA was extracted for RT-qPCR analysis for IFN-α,IFN-β and ISG54. The expression of BFRF1 or BGLF4 protein was also detected by WB using anti-Flag or anti-HA mAb, and β-actin was used to verify equal loading of protein in each lane. Statistical analysis was performed using student’s t test. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001.