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. 2020 Dec 17;11:513383. doi: 10.3389/fimmu.2020.513383

Figure 2.

Figure 2

Knockdown of BFRF1 during EBV lytic infection increases the transcriptional activations of IFN-β and ISG. (A) Hone1-EBV cells were co-transfected with reporter IFN-β-Luc, BZLF1-HA expression plasmid and pSuper-shBFRF1-retro-puro or pSuper-shRandom-retro-puro expression plasmid. At 24 h post-transfection, cells were mock-infected or infected with 100 HAU/ml SeV for 16 h. Cell lysates were then collected, and luciferase activity was measured by DLR. (B) Hone1-EBV cells were co-transfected with BZLF1-HA expression plasmid and pSuper-shBFRF1-retro-puro or pSuper-shRandom-retro-puro expression plasmid. At 24 h post-transfection, cells were mock-infected or infected with 100 HAU/ml SeV for 16 h. Cells were then lysed and RNA was extracted for reverse transcription into cDNA and qPCR analysis for the mRNA levels of IFN-β and ISG54, and RT-PCR analysis for the mRNA levels of BFRF1, BZLF1, and GAPDH. Statistical analysis was performed using Student’s t test. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001.