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BBR upregulates SIRT1 expression in 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated with BBR at various concentrations (1 and 5 μM) for 24 h. (A) Relative mRNA levels of Sirt1-7 in 3T3-L1 adipocytes measured by real-time PCR (n = 3). (B) Protein expression of SIRT1 in 3T3-L1 adipocytes was measured by Western blot analysis (C) Band intensities were quantified by normalizing to actin (n = 3). Quantitative data are presented as mean ± SD. *p < 0.05.
