FIGURE 2.

SIRT1 inhibition weakened the effect of BBR on the AMPK pathway and glucose consumption in adipocytes. 3T3-L1 adipocytes were pretreated with or without 1 μmol/L EX-527, or transfected with or without 1 × 10⁸ lentiviral shRNA expression vector as described in the Materials and Methods section and then treated with 5 μM BBR for 24 h (A,B) Glucose consumption was measured (n = 4). (C–F) Phosphorylation of LKB1, AMPK, and ACC was measured by Western blotting. Band intensities were quantified by normalizing to the corresponding total protein levels (n = 3–4). Quantitative data are presented as mean ± SD. *p < 0.05.