FIGURE 3.

SIRT1 inhibition blunted the sensitizing insulin activity of BBR in adipocytes. 3T3-L1 adipocytes pretreated with EX-527 (1 μM) or transfected with or without 1 × 10⁸ lentiviral siRNA vector were treated with 5 μM BBR in presence of 1 ng/ml TNF-α for 24 h and then stimulated with 1 nM insulin for 15 min (A,B) Adipocytes were treated with 30 μM 2-NBDG for 2 h and 1 μg/ml DAPI for 10 min. Green fluorescence of glucose in adipocytes was recorded and quantified (n = 4). (C,D) Phosphorylation of IRS-1, AKT, and actin was detected by Western blotting (E,F) Band intensities of P-IRS-1and P-AKT were quantified by normalizing to the corresponding total protein levels (n = 3). Quantitative data are presented as mean ± SD. *p < 0.05.