FIGURE 6.

BBR-induced activation of adipose tissue AMPK and insulin pathway was regulated by SIRT1. Wild Type (WT) or Sirt ^+/− (KO) C57BL/6 mice fed normal chow (NC) or high-fat diet (HFD) were treated with 50 mg/kg (BH) BBR for 2 weeks. Total protein extracted from epididymal white adipose tissue was used for Western blot analysis to measure phosphorylation of LKB1, AMPK, ACC, and AKT. Band intensities were quantified by normalizing to the corresponding total protein levels (n = 3–5). Quantitative data are presented as mean ± SD. *p < 0.05.