FIGURE 8.
Deacetylation of PGC-1α induced by BBR was abolished by inhibition of SIRT1 (A–D) 3T3-L1 adipocytes were pretreated with or without 1 μmol/L EX-527, or transfected with or without 1 × 108 lentiviral siRNA expression vector as described in the Materials and Methods section and then treated with 5 μM BBR for 24 h. Acetylation of PGC-1α was detected by immunoprecipitation and Western blot analysis. Band intensities were quantified by normalizing to the corresponding total protein levels (n = 3). (E,F) WT or Sirt +/− mice C57BL/6 fed with normal chow (NC) or high-fat diet (HFD) were treated with 50 mg/kg BBR for 2 weeks. PGC-1α acetylation in epididymal adipose tissue was measured by immunoprecipitation and Western blot analysis. Band intensities were quantified by normalizing to the corresponding total protein levels (n = 3). Quantitative data are presented as mean ± SD. *p < 0.05.