Figure 6.

Hydrogen sulfide donor and glucose-induced cell apoptosis-oxidative stress and MMP-9 activation. HRECs were analyzed for (a) cell apoptosis using an ELISA method, (b) total ROS by DCFDA method, (c) protein carbonyls by employing 2,4-dinitrophenylhydrazine, and (d) MMP-9 activity using its fluorogenic substrate. Values obtained from cells in normal glucose are considered as 100%, and are represented as mean ± SD from three different cell preparations, with each measurement performed in duplicate. *P < 0.05 versus NG; #P < 0.05 versus HG, and ^P < 0.05 versus HG/H.