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. 2020 Dec 15;5(51):33235–33241. doi: 10.1021/acsomega.0c04925

Figure 1.

Figure 1

Precipitation of DOX in buffers. DOX and DNR in buffer solutions (1 mg/mL) were incubated for 24 h at 37 °C. (A) Structure of DOX and DNR. (B) Photographs of DOX and DNR in PBS after incubation. (C) The presence or absence of precipitation of DOX and DNR in PBS (10 mM phosphate, 150 mM NaCl, pH 7.2), HBS (25 mM HEPES, 150 mM NaCl, pH 7.4), and TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.4) after incubation. (D) % precipitation of DOX in PBS after incubation, followed by four solubilization cycles with water. (E) Kinetics of DOX precipitation at different temperatures (25, 37, and 60 °C). (F) DOX precipitation in the 100 mM phosphate buffer with different pH (4.5, 6, 7, 8, and 9).