Figure 3.

Reaction modeling using DOX and hydroxylamine. DOX was incubated in PBS containing a molar equivalent of hydroxylamine for 24 h at 37 °C. Chromatograms of (A) DOX/hydroxylamine in PBS and (B) the product isolated from the blue-highlighted third peak in (A), monitoring the absorbance at 480 nm. (C) Structure of the product isolated from the third peak determined via NMR spectroscopy (NMR spectra are shown in Figure S4).