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Precipitation of DOX in the 5-FU solution. (A) Structure of 5-FU. (B) Photograph of the DOX precipitate in the 5-FU solution after incubation for 24 h at 37 °C. The DOX precipitate formed by 5-FU when dissolved with 0.1% formic acid in 50% acetonitrile and analyzed using (C) FIA–MS and (D) LC/MS. (C) Mass spectrum of the total DOX precipitate in 5-FU. (D) Chromatogram of the DOX precipitate in 5-FU, monitoring the absorbance at 480 nm.
