Table 4. Method Validation for the Two Standard Compoundsa.
precision (n = 6)b |
stability (n = 5)b |
repeatability (n = 6)b |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
analyte | calibration curve | R2 | LOD (μg/mL) | LOQ (μg/mL) | retention time | peak area | retention time | peak area | retention time | peak area | recovery (n = 6)b |
AME | Y = 34688X + 5.4043 | 0.9995 | 0.064 | 0.243 | 0.206 | 0.121 | 0.756 | 0.601 | 0.047 | 0.120 | 1.59 |
HIN | Y = 36860X + 75.613 | 0.9996 | 0.081 | 0.286 | 0.177 | 0.115 | 1.143 | 1.803 | 0.035 | 1.407 | 1.08 |
LOD, limit of detection (S/N = 3); LOQ, limit of quantification (S/N = 10).
Intraday precision, interday precision, stability, repeatability, and recovery are expressed as relative standard deviation (RSD) (%).