FIGURE 5.

PcdhgC3 knockout Brain Microvascular Endothelial Cells migrate faster in wound healing assay and their migration can be inhibited by specific MAPK-, β-catenin/Wnt- and mTOR-signaling pathway inhibitors. Wild type (WT) and PcdhgC3 knockout (KO) BMECs were seeded on µ-Dish and were grown to confluence. After 24 h serum starvation, the cells were left untreated or were treated with MAPK- (SL327, Mek1/2 inhibitor, 200 nM), Wnt- (XAV939, selective β-catenin/Wnt pathway inhibitor, 20 µM), mTOR- (Torin 2, mTOR-inhibitor, 25 nM) signaling pathways inhibitors (MAPK-Inh, Wnt-Inh, mTOR-Inh). The wells separating the cells were removed and the dishes were photographed at time 0 h. The cells were allowed to grow for 48 h into a 500-µm-space and were photographed again at 48 h. (A) The difference in area covered between 0 and 48 h was calculated and a migration rate was normalized to the control, which was arbitrarily set as 1. (B) Data are shown as mean ± standard deviation of three independent experiments. *p < 0.05, **p < 0.01.