FIGURE 8.

The effect of H₂S on the proliferative, apoptotic, and autophagic activities in the liver of NAFLD mice. (A) Representive images stained with HE and ORO (original magnification, ×400). HFD induced vacuolar degeneration of hepatocytes, disruption of normal hepatic lobules, and inflammatory cells infiltration. H₂S dramatically reduced hepatic lipid droplets and macrovesicular steatosis. (B) Liver lipid area was calculated in HE and ORO staining. (C) Representive images stained with Ki67, cleaved cas-3, and beclin-1 (original magnification, ×400). Ki67 and beclin-1 staining were reduced in HFD group, which were reversed by the administration of H₂S. The results of cleaved cas-3 staining exhibited opposite trends. (D) The indexes of proliferation, apoptosis and autophagy were determined. Data are presented as mean ± SEM (n = 6). **p < 0.01 vs. control group; ^# p < 0.05, ^## p < 0.01 vs. OA group.