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. 2020 Nov 30;11:587995. doi: 10.3389/fphar.2020.587995

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Copyright © 2020 Yang, Bai, Yu, Lu, Han, Yin and Pang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HDB reduced podocyte loss. Scale bar = 50 μm. (A–F) Representative images of WT1 immunostaining; (G) Podocyte counting. (A) SC, sham operation control group (vehicle); (B) DNM, DN model group (vehicle); (C) MET, metformin group (45 mg/kg); (D) HDBL, low-dose HDB group (110 mg/kg); (E) HDBM, medium-dose HDB group (140 mg/kg); (F) HDBH, high-dose HDB group (180 mg/kg). The data are expressed as the means ± SE for n = 8 rats/group, ^# p < 0.05, ^## p < 0.01 vs. SC, ^* p < 0.05, **p < 0.01 vs. DNM, ^△ p < 0.05 HDBH vs. MET.