Table 2.

Calculated limits of detection (LoD) and limits of quantification (LoQ) for SARS-CoV-2 routine monitoring. Concentrations are reported per L of original sample after accounting for equivalent sample volume (ESV) and recovery. The LoDs and LoQs have ranges due to the differences in ESV for each sample. Also, the LoDs and LoQs are higher for E and orf1a due to their lower ESVs.

Parameter	Sample	LoD1	N1 LoQ1	N2 LoQ1	LoD2	E LoQ2	orf1a LoQ2
qPCR Reaction	N/A	2	10	20	1	5	5
(gc)
Centricon3	WW	(3.7 ± 0.5) × 103	(1.8 ± 0.3) × 104	(3.7 ± 0.5) × 104	(9.2 ± 1.3) × 103	(4.6 ± 0.7) × 104	(4.6 ± 0.7) × 104
(gc/L)
HFUF-Centricon4	WW	(8.5 ± 3.1) × 103	(4.2 ± 1.5) × 104	(8.5 ± 3.1) × 104	(2.1 ± 0.8) × 104	(1.1 ± 0.4) × 105	(1.1 ± 0.3) × 105
(gc/L)
HFUF-Centricon4	SW/DW	(1.4 ± 0.7) × 102	(7.1 ± 3.4) × 102	(1.4 ± 0.7) × 103	N/A	N/A	N/A
(gc/L)

LoD = limit of detection; LoQ = limit of quantification; HFUF = hollow fiber ultrafiltration; gc = gene copy; WW = wastewater; SW = source water; DW = drinking water; ¹qPCR reaction with 5 μL of cDNA template; ²qPCR reaction with 1 μL of cDNA template; ³Sample concentrations adjusted for 55% recovery; ⁴Sample concentrations adjusted for 2.1% recovery.