Table 2.
Parameter | Sample | LoD1 | N1 LoQ1 | N2 LoQ1 | LoD2 | E LoQ2 | orf1a LoQ2 |
---|---|---|---|---|---|---|---|
qPCR Reaction | N/A | 2 | 10 | 20 | 1 | 5 | 5 |
(gc) | |||||||
Centricon3 | WW | (3.7 ± 0.5) × 103 | (1.8 ± 0.3) × 104 | (3.7 ± 0.5) × 104 | (9.2 ± 1.3) × 103 | (4.6 ± 0.7) × 104 | (4.6 ± 0.7) × 104 |
(gc/L) | |||||||
HFUF-Centricon4 | WW | (8.5 ± 3.1) × 103 | (4.2 ± 1.5) × 104 | (8.5 ± 3.1) × 104 | (2.1 ± 0.8) × 104 | (1.1 ± 0.4) × 105 | (1.1 ± 0.3) × 105 |
(gc/L) | |||||||
HFUF-Centricon4 | SW/DW | (1.4 ± 0.7) × 102 | (7.1 ± 3.4) × 102 | (1.4 ± 0.7) × 103 | N/A | N/A | N/A |
(gc/L) |
LoD = limit of detection; LoQ = limit of quantification; HFUF = hollow fiber ultrafiltration; gc = gene copy; WW = wastewater; SW = source water; DW = drinking water; 1qPCR reaction with 5 μL of cDNA template; 2qPCR reaction with 1 μL of cDNA template; 3Sample concentrations adjusted for 55% recovery; 4Sample concentrations adjusted for 2.1% recovery.