FIGURE 2.

CD73 deficiency facilitates NLRP3 inflammasome activation and pyroptosis of microglia in vivo. A and B, Relative mRNA expression of pyroptosis‐related genes on the third day after SCI in WT and CD73 KO mice (N = 5). C, The release of IL‐1β, IL‐6, and TNF‐α in spinal cord homogenate was measured by ELISA. D, The release of LDH was detected by LDH Assay Kit (N = 5). E and F, Representative western blotting and statistical comparison of pyroptosis‐related protein on the third day after SCI or sham surgery in WT or CD73‐deficient mice (N = 3). G, Representative two‐photon excitation images of immunofluorescence of CD68 and GSDMD acquired from WT or CD73 KO mice 3 days post‐injury or sham surgery. H and I, Immunopositive particles for GSDMD and CASP‐1 and quantification 3 days post‐injury or sham surgery in WT or CD73 KO mice (N = 5). J and K, Relative mRNA expression of pyroptosis‐related genes in BV2 cells or primary microglia with or without LPS (1μg/mL, 8 hours) treatment (N = 3). L‐N, Representative western blotting and statistical comparison of pyroptosis‐related protein in BV2 cells and primary microglia with or without LPS (1μg/mL, 8 hours) treatment (N = 3). *P < .05, **P < .01, ***P < .001. All data are shown as the mean ± SD independent experiments