FIGURE 3.

CD73 alleviates LPS‐induced NLRP3 inflammasome activation and pyroptosis in BV2 cells through A_2BAR. After small interference RNA (RNAi) or −CD73 transfection for 24 hours, BV2 cells were challenged with LPS (1μg/mL) for another 8 hours in presence of adenosine (10μM) or MRS1706 (1μM). A, The CD73 protein expression in small interference RNA or pc‐CD73 reansfected BV2 cells. B, Relative mRNA expression of CD73 in small interference RNA or pc‐CD73 transfected BV2 cells (N = 3). C, After administration of LPS combined with adenosine or MRS1706, microglial pyroptosis markers (NLRP3, ASC, CASP‐1, and GSDMD) mRNA expression were identified by RT‐PCR in CD73 downregulated or upregulated BV2 cells (N = 5). D, The release of IL‐1β, IL‐18, and TNF‐α in different cultured groups measured by ELISA (N = 5). E, The release of LDH was detected by LDH Assay Kit in different cultured groups (N = 5). F‐H, The pyroptosis‐related protein expression and statistical comparison in BV2 cells in different treated groups(N = 5). I, Representative images of immunofluorescence of GSDMD acquired from different treated BV2 cells. ^* P < .05, ^** P < .01, ^*** P < .001 versus control; ^# P < .05, ^## P < .01, ^### P < .001 versus RNAi‐CD73; ^& P < .05, ^&& P < .01 versus pc‐CD73. All data are shown as the mean ± SD independent experiments