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. 2020 Dec 31;11(1):e269. doi: 10.1002/ctm2.269

FIGURE 6.

FIGURE 6

HIF‐1α mediates CD73 upregulation in microglia. A, Relative mRNA expression of HIF‐1α in small interference RNA transfected BV2 cells (N = 3). B and C, The HIF‐1α protein expression and statistical comparison in BV2 cells with or without small interference RNA transfected (N = 3). D, The mRNA expression of HIF‐1α and CD73 in BV2 cells challenged with LPS in presence or absence of RNAi‐HIF‐1α (N = 3). *** P < .001 versus blank; # P < .05, ## P < .01 versus LPS group. E and F, The HIF‐1α and CD73 protein expression in BV2 cells challenged with LPS in presence or absence of RNAi‐HIF‐1α and statistical comparison (N = 3). *** P < .001 versus blank; # P < .05 versus LPS group. G, The mRNA expression of HIF‐1α and CD73 in WT mice 3 days post SCI, in which intraperitoneal injection of BAY87‐22430 or DMSO was carried out every day after surgery (N = 6). *** P < .001 versus Sham; # P < .05 versus SCI. H and I, The HIF‐1α and CD73 protein expression in WT mice of different groups and statistical comparison (N = 6). *** P < .001 versus Sham; ## P < .01 versus SCI. J, Immunopositive particles for CD73 3 days post‐injury in WT mice with or without BAY87‐2243 treatment. K, The mRNA expression of HIF‐1α and CD73 in BV2 cells challenged with LPS in presence or absence of RNAi‐CD73 (N = 3). ** P < .01, *** P < .001 versus blank; ## P < .01, ### P < .001 versus LPS group. L and M, The HIF‐1α and CD73 protein expression in BV2 cells challenged with LPS in presence or absence of RNAi‐CD73 and statistical comparison (N = 3). *** P < .001 versus blank; # P < .05 versus LPS group. N, The mRNA expression of HIF‐1α and CD73 in WT or CD73 KO mice 3 days post‐SCI (N = 6). ** P < .01, *** P < .001 versus WT‐Sham; # P < .05 versus WT‐SCI. O and P, The HIF‐1α and CD73 protein expression in WT or CD73 KO mice 3 days post‐SCI (N = 6). *** P < .001 versus WT‐Sham; ## P < .01 versus WT‐SCI. Q, After 24 hours pretreated with pc‐CD73, the mRNA expression of HIF‐1α and CD73 in BV2 cells with determined by RT‐PCR after challenged with LPS combined with MRS1706 (1μM) or SB203580 (10μM) for another 8 hours (N = 3). * P < .05, ** P < .01 versus control; # P < .05 versus pc‐CD73. R and S. The HIF‐1α and CD73 protein expression in different treated BV2 cells (N = 5). *** P < .001 versus control; ## P < .01 versus pc‐CD73. All data are shown as the mean ± SD independent experiments