Table. Nonsynonymous single nucleotide polymorphisms in the Kelch 13 propeller domain of clinical Plasmodium falciparum isolates collected in the Huye District, Rwanda, 2010–2019*.

Year	No. sequenced isolates	No. (%) isolates with nonsynonymous mutations	Amino acid changes and nucleotide changes
2010	75	0	Not applicable
2014	81	2 (2.5)	V555A, A626S
2015	66	3 (4.5)	P574L,† D648H, A675V†
2019	66	8 (12.1)	C469F,† G533A, V555A, R561H‡ (3×), A578S, A675V†

*Data during 2010–2015 derived from Tacoli et al. (7). 
†Candidate mutations for artemisinin resistance. 
‡Validated mutation for artemisinin resistance (4).