Figure 1.

Performance of nucleic acid amplification tests for detection of severe acute respiratory syndrome coronavirus 2 in prospectively pooled specimens. For a pool size of 8, paired individual and pooled C_t values for each individually positive sample (n = 58), in order of increasing individual C_t value. A) Pools comprising only 1 positive sample/pool. B) Pools comprising >2 positive samples/pool. The gray lines span the range of C_t values associated with a given pool. Rows without gray lines indicate individually positive samples belonging to pools that were negative by both real-time reverse transcription PCR methods. Panther Fusion is from Hologic (https://www.hologic.com). C_t, cycle threshold; LDT, laboratory-developed test.