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. 2021 Jan;27(1):92–103. doi: 10.3201/eid2701.203379

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Performance of nucleic acid amplification tests for detection of severe acute respiratory syndrome coronavirus 2 in prospectively pooled specimens. Passing-Bablok regression and Bland-Altman plots for pools of 8 containing only 1 positive sample, tested by A and B) pooled LDT versus individual LDT (n = 23) (A, B); pooled Panther Fusion versus individual LDT (n = 25) (C, D); and pooled Panther Fusion versus pooled LDT (n = 32) (E, F). For the Passing-Bablok regression plots (A, C, and E), the solid line indicates the line of regression. 95% CIs are shaded in gray. The dashed line indicates the line of identity. The slope and intercept of the regression line are reported with 95% CIs in parentheses. For the Bland-Altman plots (B, D, and F), the solid line represents the mean difference in C_t value. 95% limits of agreement are shaded in gray. Panther Fusion is from Hologic (https://www.hologic.com). C_t, cycle threshold; LDT, laboratory-developed test; LOA, limits of agreement.