Table 3. Results of 8-sample pooled testing, by testing platform and number of positive specimens per pool (n = 110) for detection of severe acute respiratory syndrome coronavirus 2*.

Pool no.	Pooled testing				Individual testing		Total no. pools
	LDT	Panther Fusion	Panther Aptima-M	Panther Aptima-350	Positive, (no. 1 PP, no. >1 PP)	Negative
1	+	+	+	+	30 (21, 9)	0	30
2	+	+	–	+	2 (1, 1)	0	2
3	+	+	–	–	0 (0, 0)	0	0
4	+	–	+	+	0 (0, 0)	0	0
5	+	–	–	+	0 (0, 0)	0	0
6	+	–	–	–	1 (1, 0)	1†	2
7	–	+	+	+	2 (2, 0)	0	2
8	–	+	–	+	1 (1, 0)	0	1
9	–	+	–	–	0 (0, 0)	1‡	1
10	–	–	+	+	2 (2, 0)	0	2
11	–	–	–	+	1 (1, 0)	1§	2
12	–	–	–	–	7 (7, 0)	61	68
No. positive pools	34	36	34	39	46 (36, 10)	–	–
No. negative pools	76	74	76	71	–	64	–
Total no. pools	110	110	110	110	–	–	110

*Panther Aptima with manufacturer-set relative light unit cutoff. Panther Aptima-350, Panther Aptima with relative light unit cutoff value >350 was considered positive; Ct, cycle threshold; LDT, laboratory-developed test; Pos, positive; RLU, relative light unit; 1 PP, 1 positive specimen in pool; >1 PP, >2 positive specimens in pool; –, negative; +, positive.

†False-positive LDT Ct value was 37.5.

‡False-positive Panther Fusion Ct value was 38.8.

§False-positive Panther Aptima-350 RLU value was 434.