Figure 1.

T cell–specific ablation of LXRβ results in T lymphocytopenia and spontaneous T cell activation. (A–J) Analysis of 6–8-wk-old CD4-CreNr1h2^fl/fl (T^ΔLXR) and Nr1h2^fl/fl (WT) littermate mice. (A) Weight gain plotted as percentage of weight at weaning (4 wk old). (B) Quantification of TCRβ⁺ T cell numbers in spleen, pLN, and liver. (C) Frequency of TCRβ⁺ T cells among CD45⁺ cells. (D and E) Representative flow cytometry plots (D) and percentages (E) of effector (CD44^hiCD62L^lo) CD4⁺ and CD8⁺ T cells. (F–H) Frequency of effector T cells producing the indicated cytokines upon ex vivo restimulation with PMA and ionomycin in the presence of brefeldin A and monensin. (F) Percentages of CD4⁺ and CD8⁺ effector T cells producing IFNγ. (G and H) Percentages of effector CD4⁺ T cells producing IL-17 (G) or IL-4 (H). (I and J) Cell numbers (I) and frequencies (J) of Foxp3⁺ T reg cells in indicated organs. Data presented as mean ± SEM (n = 5). ns, nonsignificant = P > 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Statistical significance was determined using one-way ANOVA followed by the Holm-Šídák correction. Data are representative of at least three independent experiments.