Figure S2.

Analysis of cell-intrinsic effects of Nr1h4 deficiency on peripheral T cells in mixed BM chimeras. (A) Bulk T cells from CD4-CreNr1h2^+/+ (CD45.2⁺) and CD4-CreNr1h2^fl/fl (CD45.1/.2⁺) were mixed at a 1:1 ratio and transferred into Tcrb^−/−Tcrd^−/− recipient mice. Percentages of activated (CD44^hiCD62L^lo) CD4⁺ T cells at day 7 after transfer. (B–F) CD45.2⁺ BM (BM) precursor cells from CD4-CreNr1h2^+/+, CD4-CreNr1h2^fl/+, or CD4-CreNr1h2^fl/fl animals were mixed at a 1:1 ratio with CD45.1⁺ calibrator cells from WT donors and transferred into lethally irradiated mice. Spleens of recipients were analyzed 8 wk after reconstitution. (B) Percentage of activated (CD44^hiCD62L^lo) cells among WT CD4⁺ T cells from the calibrator donor (CD45.1⁺). (C) Contribution of CD45.2⁺ cells with the indicated genotype to the effector (CD44^hiCD62L^lo) or naive (CD44^loCD62L^hi) CD4⁺ T cell pool. (D) Ratios of Foxp3⁻ to Foxp3⁺ CD4⁺T cell numbers within the CD45.2⁺ compartment. (E) Ratios of CD8⁺ to CD4⁺Foxp3⁺ T cell numbers within the CD45.2⁺ compartment. (F) Representative flow cytometry plots of Annexin V staining of effector and naive CD4⁺ T cells from the spleen of mixed chimeric mice. Data are presented as mean ± SEM (n = 4–7). ns, nonsignificant = P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Statistical significance was determined using multiple t tests (A) or one-way ANOVA (B–F) followed by the Holm-Šídák correction. Data are representative of at least two independent experiments.