Figure 4.

Identification of miR-18a target genes in cisplatin-resistant ovarian cancer. (A) qRT-PCR was performed with total RNA extracted from miR-18a-OMM or CNT-OMM transfected A2780CP20 cells. Rq (relative expression) values were calculated relative to CNT-OMM samples. (B) Western blot analysis was performed with protein extracts (50 µg) of OMM transfected cells. (C) Densitometric analysis of band intensities was performed and relative values were calculated using the intensity of β-actin as control (**P < 0.01). (D) Dual-luciferase assay was performed to assess the binding of miR-18a to the 3’UTR region of MMP-3. Experiments were performed in triplicates. Mean ± SEM is shown (*P < 0.05). (E) Western blot analysis was conducted for MMP-3 with protein extracts of ovarian cancer cell lines.