Fig 1. Nitrate and nitrite treatment did not adversely affect multiple parameters of health but nitrate treatment significantly decreased movement following a startle.

Adult zebrafish were treated with control water, sodium nitrate, or sodium nitrite and (A) nitrate and (B) nitrite concentrations were measured in newly treated fish water (fresh) and water at the end of 42-hour use (used) (n = 7–10). Fish (C) length and (D) weight was measured after 28–31 days of treatment (n = 18–33). At 14–17 days of treatment the (E) distance and (F) velocity zebrafish traveled was quantified in the voluntary swimming assay (n = 39–42) or (G) the response to five sequential acoustic startles (as taps against the fish tank) was quantified (n = 84–90). (A-G) Bars represent the mean ± SEM.