Fig 3. Nitrate and nitrite treatment increased mild anxiogenic-like behavior in zebrafish.

Adult zebrafish were treated with control water, sodium nitrate, or sodium nitrite for 14–17 days. (A) Movement in a novel tank was recorded and the percent of time spent in the bottom, middle and top zones of tank are indicated (n = 83–89). (B-D) Concentrations of hormones were measured in whole fish by ELISA (n = 12). (A-D) Bars represent the mean ± SEM.