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. 2020 Dec 31;15(12):e0240070. doi: 10.1371/journal.pone.0240070

Fig 5. Nitrate and nitrite treatment significantly altered the abundance of some brain metabolites.

Fig 5

Adult zebrafish were treated with control water, sodium nitrate, or sodium nitrite for 31 days and brain metabolites were measured using untargeted LC-MS/MS. (A) Partial least squares discriminant analysis (PLS-DA) scored plot demonstrates spatial clustering and separation between treatment groups when considering all the annotated compounds. (B) PLS-DA variable importance in projection (VIP) graph of the most relevant 30 features (when considering the three treatments). Colored boxes at right indicate the mean relative concentrations of the corresponding metabolite in each treatment group under study. Red color indicates higher abundance, while green color indicates lower abundance. The PLS-DA model display 95% confidence region. Abbreviations: dADP (deoxyadenosine diphosphate), NAM (N-acetyl-L-methionine), 3-Hydroxybenzo (3-Hydroxybenzoic acid), 1-Aminocyclopr (1-Aminocyclopropane-1-carboxylate), 3PG (3-Phosphoglyceric acid), NADP (Nicotinamide adenine dinucleotide phosphate), ARA (Arachidonic acid), NAD (Nicotinamide adenine dinucleotide), EPA (Eicosapentanoic acid), 12-Hydroxydode (12-Hydroxydodecanoic acid), MMA (Methylmalonic acid), 2-Hydroxybutyrate (2-Hydroxybutyric acid).