Fig. 1.

Cation-dependent astrocyte adhesion is reduced by EGFRvIII. (A) Western blot of indicated genotypes for Pten, EGFR and actin. (B) Shear stress at which 50% of the population detaches, i.e. adhesion strength of τ₅₀, is plotted for the indicated genotypes. Cells assayed in buffer with (solid bars) and without (hatched bars) cation-containing medium are shown. *P<0.05 and **P<0.01 by paired Student’s t-test (n=4 per genotype and condition; solid and dashed lines indicate comparisons of cells with and without cation-containing medium, respectively).