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. 2020 Dec 31;15(12):e0230939. doi: 10.1371/journal.pone.0230939

Fig 3. Localization of SPE-50::mCherry and SPE-47::GFP translational reporters in male gonads.

Fig 3

The fluorescent images are 3D reconstructions of a stack of images. (A) The double reporter strain constructs and imaging in blue (nuclei), green (spe-47::GFP), and red (spe-50::mCherry). A region of the gonad in the merge image shown by the box is enlarged to give better detail of the localization. In this enlargement, only the middle of the 3D reconstruction is shown to give better understanding of colocalization. (B) Imaging from the unlabeled wild-type strain for comparison. In both the reporter images and the wild-type control, there are remnants of the intestine present. The intestine is highly autofluorescent in green and red.