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. 2020 Dec 31;15(12):e0244549. doi: 10.1371/journal.pone.0244549

Fig 5. Carboplatin response of EOC spheroids cultured with 2% matrigel in ultra-low attachment wells.

Fig 5

3D spheroids of TOV3041G (A), TOV112D (B), OV90 (C) and OV866(2) (D) were formed and cultured in concave-bottom ULA plates in the presence of 2% Matrigel for 4 days (Ctrl Day 4) or treated at day 2 with 300 μM of carboplatin and assessed at day 4 (Carbo 300 Day 4). Top panels are representative microscopic bright field images of each condition. Bar graphs represent cell survival analysed by flow cytometry of control (light grey) and treated (dark grey) spheroids. Data are shown as the mean ± SEM of three independent experiments. * p<0.05; ** p<0.005 (Student’s t-test).