Table 1. Primer sequences for miRNA reverse transcription quantitative PCR.
Primer name | Sequence (5' to 3') |
---|---|
mmu-let-7a-5p-F | ACACTCCAGCTGGGTGAGGTAGTAGGTTGT |
mmu-miR-10a-5p-F | ACACTCCAGCTGGGTACCCTGTAGATCCGAAT |
mmu-miR-122-5p -F | ACACTCCAGCTGGGTGGAGTGTGACAATGGTG |
mmu-miR-143-3p-F | ACACTCCAGCTGGGTGAGATGAAGCACTGTAG |
mmu-miR-150-5p-F | ACACTCCAGCTGGGTCTCCCAACCCTTGTACC |
mmu-miR-155-5p-F | ACACTCCAGCTGGGTTAATGCTAATTGTGAT |
mmu-miR-29a-3p-F | ACACTCCAGCTGGGTAGCACCATCTGAAATC |
*miRNA-R | CTCAACTGGTGTCGTGGA |
U6-F | CTCGCTTCGGCAGCACA |
U6-R | AACGCTTCACGAATTTGCGT |
*miRNA-R serves as the universal reverse primer for all miRNAs in the present study.