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. 2020 Dec 24;2020:8824805. doi: 10.1155/2020/8824805

Figure 1.

Figure 1

CRISPR/Cas9-mediated B7-H3 KO reduces proliferation of H3255 and HCC827 strains. (a) Representative flow cytometry analysis for B7-H3 expression in H3255 KO and HCC827 KO cells. Grey shadow, IgG isotype control; grey and black lines correspond to mock and KO cells stained with anti-B7-H3, respectively. (b) Representative western blot analysis for B7-H3 expression in wild-type (WT), B7-H3 KO, and mock H3255 and HCC827 cells as indicated. (c, d) The fold change proliferation of B7-H3 KO and mock H3255 (c) and HCC827 (d) cells was determined by WST-8/CCK-8 at 0 h, 24 h, 48 h, and 72 h after cell culturing.