HL-60/dnr cells (resistant to daunorubicin) (800,000/ml dnr-free, RPMI-1640 medium, 10% FBS) were exposed to 10 μM SACLAC (DMSO vehicle) or DMSO (control) for 24 hr. Cells were then harvested by centrifugation, washed three times in PBS, and subjected to lipidomic evaluation by LC/ESI/MS/MS. A. Ceramide molecular species. B. Dihydroceramide molecular species. Viability in SACLAC-treated cells was 80% at harvest. Data are expressed as fold change compared with untreated controls. Data from Kao L-P, et al., J. Lipid Res., 60: 1590–1602, 2019, and reproduced with permission from the publisher.