Statistical results used to determine model selection for mixed-effects models. Full models included estrous stage (proestrus, estrus, Metestrus, diestrus 1, and diestrus 2), recording condition (dyad, elicited, and isolation), and their interaction as fixed effects and individual rat as the random effect. Reduced models did not include the interaction term but all other fixed and random effects. ANOVAs were used to test full versus reduced models. No significance resulted in selection of reduced models. Numbers were rounded to the third decimal place.

ANOVAs of full vs reduced models for acoustic variables with nonsignificant interaction terms.
Acoustic Parameter	npar	AIC	BIC	logLik	deviance	Chisq	Df	Pr(>Chisq)
Frequency Bandwidth	8	86042.014	86100.948	−43013.007	86026.014
	12	86046.176	86134.577	−43011.088	86022.176	3.837	4	0.428
Slope	8	166593.567	166652.501	−83288.783	166577.567
	12	166593.189	166681.590	−83284.595	166569.189	8.378	4	0.079
Frequency Standard Deviation	8	58644.592	58703.526	−29314.296	58628.592
	12	58650.211	58738.612	−29313.106	58626.211	2.381	4	0.666
Sinuosity	8	25827.782	25886.716	−12905.891	25811.782
	12	25833.094	25921.495	−12904.547	25809.094	2.688	4	0.611